Oct4 upregulates osteopontin via Egr1 and is associated with poor outcome in human lung cancer.

ABSTRACT:

Background

Roles of cancer stem cells and early growth response gene 1 (Egr1) in carcinogenesis have been extensively studied in lung cancer. However, the role of Egr1 in the metastasis of lung cancer remains undetermined, especially in regard to stem cell-related pathways.

Methods

Egr1, osteopontin (OPN) and Oct4 expression in human lung cancer was determined by performing immunohistochemistry. Immunoblotting, ELISA, luciferase reporter assay, chromatin immunoprecipitation assay and RT-PCR were performed to validate the regulation of Oct4-Egr1-OPN axis. Moreover, the effect of Oct4-Egr1-OPN axis on lung cancer progression was evaluated by cell migration assay and mice study.

Results

We detected Oct4, Egr1, and OPN expression in clinical specimens from 79 lung cancer

SUBMITTER: Feng YH 

PROVIDER: S-EPMC6688208 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature