Neuroinflammatory astrocytes generated from cord blood-derived human induced pluripotent stem cells.

ABSTRACT:

Background

Astrocytes respond to central nervous system (CNS) injury and disease by transforming to a reactive astrogliosis cell state that can contribute to either CNS dysfunction or repair. Neuroinflammation is a powerful driver of a harmful A1 astrogliosis phenotype associated with in vitro neurotoxicity and histopathology in human neurodegenerative diseases. Here we report a protocol for the rapid development of a human cell culture model of neuroinflammatory astrogliosis using induced pluripotent stem cells (iPSCs).

Methods

Using RNA sequencing and in vitro cell assays, we measured transcriptional and cellular effects of chronic exposure of human iPSC-derived astrocytes to the cytokines TNFα (tumor necrosis factor alpha) or IL-1β (interleukin-1 beta).

Results

We

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC6688278 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature