Quantitative proteomic analysis reveals AK2 as potential biomarker for late normal tissue radiotoxicity.

ABSTRACT:

Background

Biomarkers for predicting late normal tissue toxicity to radiotherapy are necessary to personalize treatments and to optimize clinical benefit. Many radiogenomic studies have been published on this topic. Conversely, proteomics approaches are not much developed, despite their advantages.

Methods

We used the isobaric tags for relative and absolute quantitation (iTRAQ) proteomic approach to analyze differences in protein expression levels in ex-vivo irradiated (8 Gy) T lymphocytes from patients with grade ≥ 2 radiation-induced breast fibrosis (grade ≥ 2 bf+) and patients with grade < 2 bf + after curative intent radiotherapy. Patients were selected from two prospective clinical trials (COHORT and PHRC 2005) and were used as discovery and confirmation cohorts.

Res

SUBMITTER: Lacombe J 

PROVIDER: S-EPMC6688300 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature