Mass Cytometry Discovers Two Discrete Subsets of CD39^-Treg Which Discriminate MGUS From Multiple Myeloma.

ABSTRACT: Multiple Myeloma (MM) is preceded by the clinically stable condition monoclonal gammopathy of undetermined significance (MGUS). Critical immune events that discriminate MGUS from newly diagnosed MM (ND)MM patients remain unknown, but may involve changes in the regulatory T cell (Treg) compartment that favor myeloma growth. To address this possibility, we used mass cytometry and the unsupervised clustering algorithm Flow self-organizing map (FlowSOM) to interrogate the distribution of multiple subsets within CD25⁺CD127^low/negTreg in matched bone marrow (BM) and peripheral blood (PB) of MGUS and NDMM patients. Both mass cytometry and flow cytometry confirmed a trend toward prevalence of CD39^-Treg within the Treg compartment in BM and PB of NDMM patients compa

SUBMITTER: Marsh-Wakefield F 

PROVIDER: S-EPMC6688400 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature