Ultrasonically-guided flow focusing generates precise emulsion droplets for high-throughput single cell analyses.

ABSTRACT: Emulsion-based techniques have dramatically advanced our understanding of single-cell biology and complex single-cell features over the past two decades. Most approaches for precise single cell isolation rely on microfluidics, which has proven highly effective but requires substantial investment in equipment and expertise that can be difficult to access for researchers that specialize in other areas of bioengineering and molecular biotechnology. Inspired by the robust droplet generation technologies in modern flow cytometry instrumentation, here we established a new platform for high-throughput isolation of single cells within droplets of tunable sizes by combining flow focusing with ultrasonic vibration for rapid and effective droplet formation. Application of ultrasonic pressure waves to

SUBMITTER: Lagerman CE 

PROVIDER: S-EPMC6688500 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature