Single-cell transcriptome analysis of CD8 ⁺ T-cell memory inflation.

ABSTRACT: Background: Persistent viruses such as murine cytomegalovirus (MCMV) and adenovirus-based vaccines induce strong, sustained CD8 ⁺ T-cell responses, described as memory "inflation". These retain functionality, home to peripheral organs and are associated with a distinct transcriptional program. Methods: To further define the nature of the transcriptional mechanisms underpinning memory inflation at different sites we used single-cell RNA sequencing of tetramer-sorted cells from MCMV-infected mice, analyzing transcriptional networks in virus-specific populations in the spleen and gut intra-epithelial lymphocytes (IEL). Results: We provide a transcriptional map of T-cell memory and define a module of gene expression, which distinguishes memory inflation in splee

SUBMITTER: Highton AJ 

PROVIDER: S-EPMC6688724 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature