A single-nuclei RNA sequencing study of Mendelian and sporadic AD in the human brain.

ABSTRACT:

Background

Alzheimer's disease (AD) is the most common form of dementia. This neurodegenerative disorder is associated with neuronal death and gliosis heavily impacting the cerebral cortex. AD has a substantial but heterogeneous genetic component, presenting both Mendelian and complex genetic architectures. Using bulk RNA-seq from the parietal lobes and deconvolution methods, we previously reported that brains exhibiting different AD genetic architecture exhibit different cellular proportions. Here, we sought to directly investigate AD brain changes in cell proportion and gene expression using single-cell resolution.

Methods

We generated unsorted single-nuclei RNA sequencing data from brain tissue. We leveraged the tissue donated from a carrier of a Mendelian genetic mutatio

SUBMITTER: Del-Aguila JL 

PROVIDER: S-EPMC6689177 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature