LC-MS/MS based 25(OH)D status in a large Southern European outpatient cohort: gender- and age-specific differences.

ABSTRACT:

Background

Developed countries have a high prevalence of vitamin D deficiency. In previous studies, 25(OH)D was predominantly measured by immunoassays. The present study assessed serum 25(OH)D in a very large Southern European outpatient cohort by liquid chromatography tandem mass spectrometry (LC-MS/MS).

Materials and methods

74,235 serum 25(OH)D results generated under routine conditions between 2015 and 2016 were extracted from the laboratory information system of the Department of Clinical Pathology at Bolzano Hospital (Italy). In 3801 cases, parathyroid hormone (PTH) was requested in parallel. Serum 25(OH)D was measured by a NIST-972 aligned commercial LC-MS/MS method. The distribution of serum 25(OH)D concentrations in males and females of different age groups, the pre

SUBMITTER: Giuliani S 

PROVIDER: S-EPMC6689275 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature