Molecular cloning and functional analysis of a Δ¹²-fatty acid desaturase from the Antarctic microalga Chlamydomonas sp. ICE-L.

ABSTRACT: Chlamydomonas sp. ICE-L, which can thrive in extreme environments of the Antarctic, could represent a promising alternative for polyunsaturated fatty acid (PUFA) production. A new Δ¹²-fatty acid desaturase (FAD)-encoding gene (Δ ¹² CiFAD), 1269 bp in size, was cloned from Chlamydomonas sp. ICE-L. Bioinformatics analysis showed that Δ ¹² CiFAD-encoded protein was homologous to known FADs with conserved histidine motifs, and localized to the chloroplast. Functional analysis of Δ ¹² CiFAD indicated that recombinant Synechococcus 6803 expressing Δ¹²CiFAD could accumulate C18:2, whereas recombinant Saccharomyces cerevisiae expressing this enzyme could

SUBMITTER: He Y 

PROVIDER: S-EPMC6689313 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature