Shaping the lipid composition of bacterial membranes for membrane protein production.

ABSTRACT:

Background

The overexpression and purification of membrane proteins is a bottleneck in biotechnology and structural biology. E. coli remains the host of choice for membrane protein production. To date, most of the efforts have focused on genetically tuning of expression systems and shaping membrane composition to improve membrane protein production remained largely unexplored.

Results

In E. coli C41(DE3) strain, we deleted two transporters involved in fatty acid metabolism (OmpF and AcrB), which are also recalcitrant contaminants crystallizing even at low concentration. Engineered expression hosts presented an enhanced fitness and improved folding of target membrane proteins, which correlated with an altered membrane fluidity. We demonstrated the scope of this approach by ov

SUBMITTER: Kanonenberg K 

PROVIDER: S-EPMC6689329 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature