T-GRASP, a targeted GRASP for assessing neuronal connectivity.

ABSTRACT:

Background

Understanding how behaviors are generated by neural circuits requires knowledge of the synaptic connections between the composite neurons. Methods for mapping synaptic connections, such as electron microscopy and paired recordings, are labor intensive and alternative methods are thus desirable.

New method

Development of a targeted GFP Reconstitution Across Synaptic Partners(GRASP) method, t-GRASP, for assessing neural connectivity is described.

Results

Numerous different pre-synaptic and post-synaptic/dendritic proteins were tested for enhancing the specificity of GRASP signal to synaptic regions. Pairing of both targeted pre- and post-t-GRASP constructs resulted in strong preferential GRASP signal in synaptic regions in Drosophila larval sensory neurons, l

SUBMITTER: Shearin HK 

PROVIDER: S-EPMC6689385 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature