The effect of platelet lysate in culture of PDLSCs: an in vitro comparative study.

ABSTRACT:

Background

Cellular therapy clinical applications require large-scale production of stem cells. Therefore, abundance, ease of isolation, and proliferative potential are the most important factors in choosing the appropriate source of cells for transplantation studies. Multipotent stem cells obtained from periodontal ligament (PDL) can be used in periodontal tissue regeneration. In this study, we aimed to evaluate and compare the characteristics of periodontal ligament stem cells (PDLSCs), extracted by either enzymatic digestion or explant methods, and expanded using two different serum types: fetal bovine serum (FBS) and xeno-free platelet lysate (PL).

Methods

Expanded PDLSCs were assessed for their proliferation capacity, surface markers expression, colony formation, differ

SUBMITTER: Abuarqoub DA 

PROVIDER: S-EPMC6689390 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature