A Derived Positional Mapping of Inhibitory Subtypes in the Somatosensory Cortex.

ABSTRACT: Obtaining a catalog of cell types is a fundamental building block for understanding the brain. The ideal classification of cell-types is based on the profile of molecules expressed by a cell, in particular, the profile of genes expressed. One strategy is, therefore, to obtain as many single-cell transcriptomes as possible and isolate clusters of neurons with similar gene expression profiles. In this study, we explored an alternative strategy. We explored whether cell-types can be algorithmically derived by combining protein tissue stains with transcript expression profiles. We developed an algorithm that aims to distribute cell-types in the different layers of somatosensory cortex of the developing rat constrained by the tissue- and cellular level data. We found that the spatial distributi

SUBMITTER: Keller D 

PROVIDER: S-EPMC6691028 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature