Improved mutant function prediction via PACT: Protein Analysis and Classifier Toolkit.

ABSTRACT:

Motivation

Deep mutational scanning experiments have enabled the measurement of the sequence-function relationship for thousands of mutations in a single experiment. The Protein Analysis and Classifier Toolkit (PACT) is a Python software package that marries the fitness metric of a given mutation within these experiments to sequence and structural features enabling downstream analyses. PACT enables the easy development of user sharable protocols for custom deep mutational scanning experiments as all code is modular and reusable between protocols. Protocols for mutational libraries with single or multiple mutations are included. To exemplify its utility, PACT assessed two deep mutational scanning datasets that measured the tradeoff of enzyme activity and enzyme stability.

Results

SUBMITTER: Klesmith JR 

PROVIDER: S-EPMC6691332 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature