Drug metabolite synthesis by immobilized human FMO3 and whole cell catalysts.

ABSTRACT:

Background

Sufficient reference standards of drug metabolites are required in the drug discovery and development process. However, such drug standards are often expensive or not commercially available. Chemical synthesis of drug metabolite is often difficulty due to the highly regio- and stereo-chemically demanding. The present work aims to construct stable and efficient biocatalysts for the generation of drug metabolites in vitro.

Result

In this work, using benzydamine as a model drug, two easy-to-perform approaches (whole cell catalysis and enzyme immobilization) were investigated for the synthesis of FMO3-generated drug metabolites. The whole cell catalysis was carried out by using cell suspensions of E. coli JM109 harboring FMO3 and E. coli BL21 harboring GDH (glucose de

SUBMITTER: Gao C 

PROVIDER: S-EPMC6691536 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature