Enhanced cellulase production by decreasing intercellular pH through H⁺-ATPase gene deletion in Trichoderma reesei RUT-C30.

ABSTRACT:

Background

Cellulolytic enzymes produced by Trichoderma reesei are widely used for the industrial production of biofuels and chemicals from lignocellulose. We speculated that intracellular pH during the fermentation process can affect cellulase induction.

Results

In this study, two H⁺-ATPase genes, tre76238 and tre78757, were first identified in T. reesei. Deletion of tre76238 and tre78757 in T. reesei RUT-C30 confirmed that tre76238 has a major function in maintaining intracellular pH, whereas tre78757 has a minor function. The tre76238 deletion strain Δ76238 displayed a high level of cellulase production using cellulase-repressive glucose as a sole carbon source, along with intracellular acid

SUBMITTER: Liu P 

PROVIDER: S-EPMC6691542 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature