Evaluation of serum extracellular vesicles as noninvasive diagnostic markers of glioma.

ABSTRACT: Rationale: Glioma is the most common malignant primary brain tumor in the central nervous system (CNS). The lack of reliable noninvasive diagnostic and prognostic methods is one of the main reasons for the high mortality of glioma. Serum has become a useful biomarker for the diagnosis and prognosis prediction of glioma because extracellular vesicles (EVs) carry molecular components from their parental cells. Methods: To detect EVs and perform molecular analysis of serum EVs, we established and optimized a microbead-assisted method based on flow cytometry and estimated the efficacy of EGFR protein expression and NLGN3 and PTTG1 mRNA in serum EVs from glioma patients (n=23) and healthy individuals (n=12). We evaluated the ability of EGFR⁺ EVs to differe

SUBMITTER: Wang H 

PROVIDER: S-EPMC6691576 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature