RNA-silencing nanoprobes for effective activation and dynamic imaging of neural stem cell differentiation.

ABSTRACT: To achieve the clinical potential of neural stem cells (NSCs), it is crucial to activate NSC differentiation into neurons and simultaneously monitor the process of NSC differentiation. However, there are many challenges associated with regulating and tracking NSC differentiation. Methods: We developed a redox-responsive multifunctional nanocomplex with a disulfide bond-cvNC-for the delivery of siRNAs to induce NSC differentiation through sequence-specific RNA interference (RNAi) and real-time imaging of sequential mRNA expression during differentiation. The stability and specificity of cvNCs were studied in vitro. Controlled release of siRNA, gene silencing efficiency, as well as real-time imaging of cvNCs on Tubb3 and Fox3 mRNAs during NSC differentiation were

SUBMITTER: Zhang R 

PROVIDER: S-EPMC6691577 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature