Development of a Molecular Marker Linked to the A4 Locus and the Structure of HD Genes in Pleurotus eryngii.

ABSTRACT: Allelic differences in A and B mating-type loci are a prerequisite for the progression of mating in the genus Pleurotus eryngii; thus, the crossing is hampered by this biological barrier in inbreeding. Molecular markers linked to mating types of P. eryngii KNR2312 were investigated with randomly amplified polymorphic DNA to enhance crossing efficiency. An A4-linked sequence was identified and used to find the adjacent genomic region with the entire motif of the A locus from a contig sequenced by PacBio. The sequence-characterized amplified region marker 7-2₂₉₉ distinguished A4 mating-type monokaryons from KNR2312 and other strains. A BLAST search of flanked sequences revealed that the A4 locus had a general feature consisti

SUBMITTER: Lee SH 

PROVIDER: S-EPMC6691818 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature