Hippocampal transcriptomic responses to enzyme-mediated cellular dissociation.

ABSTRACT: Single-neuron gene expression studies may be especially important for understanding nervous system structure and function because of the neuron-specific functionality and plasticity that defines functional neural circuits. Cellular dissociation is a prerequisite technical manipulation for single-cell and single cell-population studies, but the extent to which the cellular dissociation process affects neural gene expression has not been determined. This information is necessary for interpreting the results of experimental manipulations that affect neural function such as learning and memory. The goal of this research was to determine the impact of cellular dissociation on brain transcriptomes. We compared gene expression of microdissected samples from the dentate gyrus (DG), CA3, and CA1 su

SUBMITTER: Harris RM 

PROVIDER: S-EPMC6692227 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature