A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier.

ABSTRACT: In this work we model the glomerular filtration barrier, the structure responsible for filtering the blood and preventing the loss of proteins, using human podocytes and glomerular endothelial cells seeded into microfluidic chips. In long-term cultures, cells maintain their morphology, form capillary-like structures and express slit diaphragm proteins. This system recapitulates functions and structure of the glomerulus, including permselectivity. When exposed to sera from patients with anti-podocyte autoantibodies, the chips show albuminuria proportional to patients' proteinuria, phenomenon not observed with sera from healthy controls or individuals with primary podocyte defects. We also show its applicability for renal disease modeling and drug testing. A total of 2000 independent chips w

SUBMITTER: Petrosyan A 

PROVIDER: S-EPMC6692336 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature