Characterization of H/D exchange in type 1 pili by proton-detected solid-state NMR and molecular dynamics simulations.

ABSTRACT: Uropathogenic Escherichia coli invades and colonizes hosts by attaching to cells using adhesive pili on the bacterial surface. Although many biophysical techniques have been used to study the structure and mechanical properties of pili, many important details are still unknown. Here we use proton-detected solid-state NMR experiments to investigate solvent accessibility and structural dynamics. Deuterium back-exchange at labile sites of the perdeuterated, fully proton back-exchanged pili was conducted to investigate hydrogen/deuterium (H/D) exchange patterns of backbone amide protons in pre-assembled pili. We found distinct H/D exchange patterns in lateral and axial intermolecular interfaces in pili. Amide protons protected from H/D exchange in pili are mainly located in the core region of

SUBMITTER: Hwang S 

PROVIDER: S-EPMC6692446 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature