Characterization of a phospholipid-regulated β-galactosidase from Akkermansia muciniphila involved in mucin degradation.

ABSTRACT: The gut microbe Akkermansia muciniphila is important for the human health as the occurrence of the organism is inversely correlated with different metabolic disorders. The metabolism of the organism includes the degradation of intestinal mucins. Thus, the gut health-promoting properties are not immediately obvious and mechanisms of bacteria-host interactions are mostly unclear. In this study, we characterized a novel extracellular β-galactosidase (Amuc_1686) with a preference for linkages from the type Galβ1-3GalNAc. Additionally, Amuc_1686 possesses a discoidin-like domain, which enables the interaction with anionic phospholipids. We detected a strong inhibition by phosphatidylserine, phosphatidylglycerol, phosphatidic acid, and lysophosphatidic acid while phosphatidylcholine and phosphat

SUBMITTER: Kosciow K 

PROVIDER: S-EPMC6692548 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature