MicroRNA-29b-3p reduces intestinal ischaemia/reperfusion injury via targeting of TNF receptor-associated factor 3.

ABSTRACT:

Background and purpose

The microRNA miR-29b-3p shows important roles in regulating apoptosis and inflammation. However, its effects on intestinal ischaemia/reperfusion (II/R) injury have not been reported. Here we have investigated the functions of miR-29b-3p on II/R injury on order to find drug targets to treat the injury.

Experimental approach

Two models - in vitro hypoxia/reoxygenation (H/R) of IEC-6 cells; in vivo, II/R injury in C57BL/6 mice were used. Western blotting and dual-luciferase reporter assays were used and mimic and siRNA transfection tests were applied to assess the effects of miR-29b-3p on II/R injury via targeting TNF receptor-associated factor 3 (TRAF3).

Key results

The H/R procedure decreased cell viability and promoted inflammation and apoptosis

SUBMITTER: Dai Y 

PROVIDER: S-EPMC6692574 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature