Salinomycin exerts anti-colorectal cancer activity by targeting the β-catenin/T-cell factor complex.

ABSTRACT:

Background and purpose

Salinomycin is a well-known inhibitor of human cancer stem cells (CSCs). However, the molecular mechanism(s) by which salinomycin targets colorectal CSCs is poorly understood. Here, we have investigated underlying antitumour mechanisms of salinomycin in colorectal cancer cells and three tumour models.

Experimental approach

The inhibitory effect of salinomycin on the Wnt/β-catenin pathway was analysed with the SuperTopFlash reporter system. The mRNA expression of Wnt target genes was evaluated with real-time PCR. Effects of salinomycin on β-catenin/TCF4E interaction were examined using co-immunoprecipitation and an in vitro GST pull-down assay. Cell proliferation was determined by BrdU incorporation and soft agar colony formation assay. The stemness of

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6692576 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature