A simple method to monitor hepatic gluconeogenesis and triglyceride synthesis following oral sugar tolerance test in obese adolescents.

ABSTRACT: Hepatic energy metabolism is a key element in many metabolic diseases. Hepatic anaplerosis provides carbons for gluconeogenesis (GNG) and triglyceride (TG) synthesis. We aimed to optimize a protocol that measures hepatic anaplerotic contribution for GNG, TG synthesis, and hepatic pentose phosphate pathway (PPP) activity using a single dose of oral [U^-13C₃]glycerol paired with an oral sugar tolerance test (OSTT) in a population with significant insulin resistance. The OSTT (75 g glucose + 25 g fructose) was administered to eight obese adolescents with polycystic ovarian syndrome (PCOS) followed by ingestion of [U-¹³C₃]glycerol at t = 180 or t = 210 min. ¹³C-labeling patterns of serum glucose and TG-glycerol were determined

SUBMITTER: Carreau AM 

PROVIDER: S-EPMC6692761 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature