VEGF-C induced by TGF- β1 signaling in gastric cancer enhances tumor-induced lymphangiogenesis.

ABSTRACT:

Background

The role of TGF-β1 in lymph node metastasis and lymphangiogenesis, one of the most important steps of gastric cancer dissemination, is largely unknown. The goal of this study was to investigate the role of TGF-β1 signaling and its molecular mechanisms involved in lymphangiogenesis of gastric cancer.

Methods

Two gastric cell line models, MKN45 and KATOIII, were selected for this study. The protein expression of TGF-β1 pathway molecules and VEGF-C were examined with western blot, or ELISA according to TGF-β1 treatment. To explore whether Smad3 binds to the specific DNA sequences in the VEGFC promoter, we performed an electrophoretic mobility shift assay. Lymphatic tube forming assay and gastric cancer xenograft mouse models were also used to elucidate the effect of

SUBMITTER: Pak KH 

PROVIDER: S-EPMC6692962 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature