Development and evaluation of a rapid and sensitive RPA assay for specific detection of Vibrio parahaemolyticus in seafood.

ABSTRACT:

Background

Vibrio parahaemolyticus (V. parahaemolyticus) is a leading cause of food poisoning and is of great importance to public health due to the frequency and seriousness of the diseases. The simple, timely and efficient detection of this pathogen is a major concern worldwide. In this study, we established a simple and rapid method based on recombinase polymerase amplification (RPA) for the determination of V. parahaemolyticus. According to the gyrB gene sequences of V. parahaemolyticus available in GenBank, specific primers and an exo probe were designed for establishing real-time recombinase polymerase amplification (real-time RPA).

Results

The real-time RPA reaction was performed successfully at 38 °C, and results were obtained within 20 min. The method only detected

SUBMITTER: Geng Y 

PROVIDER: S-EPMC6693139 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature