Renal temperature reduction progressively favors mitochondrial ROS production over respiration in hypothermic kidney preservation.

ABSTRACT:

Background

Hypothermia, leading to mitochondrial inhibition, is widely used to reduce ischemic injury during kidney preservation. However, the exact effect of hypothermic kidney preservation on mitochondrial function remains unclear.

Methods

We evaluated mitochondrial function [i.e. oxygen consumption and production of reactive oxygen species (ROS)] in different models (porcine kidney perfusion, isolated kidney mitochondria, and HEK293 cells) at temperatures ranging 7-37 °C.

Results

Lowering temperature in perfused kidneys and isolated mitochondria resulted in a rapid decrease in oxygen consumption (65% at 27 °C versus 20% at 7 °C compared to normothermic). Decreased oxygen consumption at lower temperatures was accompanied by a reduction in mitochondrial ROS productio

SUBMITTER: Hendriks KDW 

PROVIDER: S-EPMC6693148 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature