Extreme-QTL mapping of monepantel resistance in Haemonchus contortus.

ABSTRACT:

Background

Haemonchus contortus, a gastrointestinal nematode parasite of sheep, is mainly controlled by anthelmintics; the occurrence of anthelmintic resistance leads to treatment failures and increases economic burden. Because molecular mechanisms involved in drug resistance can be elucidated by genomic studies, an extreme quantitative trait locus (X-QTL) mapping approach was used to identify co-segregation of the resistance phenotype with genetic markers to detect the genome-wide variants associated with monepantel resistance in H. contortus.

Methods

A cross between H. contortus isolates using parental susceptible (Par-S) males and monepantel resistant (Par-R) females resulted in SR progeny, while reciprocal cross resulted in RS progeny. Pools (n = 30,000) of infective lar

SUBMITTER: Niciura SCM 

PROVIDER: S-EPMC6693152 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature