Enhanced acid-stress tolerance in Lactococcus lactis NZ9000 by overexpression of ABC transporters.

ABSTRACT:

Background

Microbial cell factories are widely used in the production of acidic products such as organic acids and amino acids. However, the metabolic activity of microbial cells and their production efficiency are severely inhibited with the accumulation of intracellular acidic metabolites. Therefore, it remains a key issue to enhance the acid tolerance of microbial cells. In this study, we investigated the effects of four ATP-binding cassette (ABC) transporters on acid stress tolerance in Lactococcus lactis.

Results

Overexpressing the rbsA, rbsB, msmK, and dppA genes exhibited 5.8-, 12.2-, 213.7-, and 5.2-fold higher survival rates than the control strain, respectively, after acid shock for 3 h at pH 4.0. Subsequently, transcriptional profile alterations in recombinant str

SUBMITTER: Zhu Z 

PROVIDER: S-EPMC6693162 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature