The dynamics of FTO binding and demethylation from the m⁶A motifs.

ABSTRACT: N⁶-methyladenosine (m⁶A) is considered as a reversible RNA modification occurring more frequently on the GAC than AAC context in vivo, which regulates post-transcriptional gene expression in mammalian cells. m⁶A 'writers' METTL3 and METTL14 demonstrate a strong preference for binding AC-containing motifs in living cells. However, this evidence is currently lacking for m⁶A erasers, leaving the dynamics of the internal m⁶A modification under debate recently. We analysed three recently published FTO CLIP-seq data sets and two generated in this study, one of the two known m⁶A 'erasers'. FTO binding peaks from all cell lines contain RRACH motifs. Only those from K562, 3T3-L1and HeLa cells were enriched in AC-contain

SUBMITTER: Li Y 

PROVIDER: S-EPMC6693534 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature