Landscape of the Plasmodium Interactome Reveals Both Conserved and Species-Specific Functionality.

ABSTRACT: Malaria represents a major global health issue, and the identification of new intervention targets remains an urgent priority. This search is hampered by more than one-third of the genes of malaria-causing Plasmodium parasites being uncharacterized. We report a large-scale protein interaction network in Plasmodium schizonts, generated by combining blue native-polyacrylamide electrophoresis with quantitative mass spectrometry and machine learning. This integrative approach, spanning 3 species, identifies >20,000 putative protein interactions, organized into 600 protein clusters. We validate selected interactions, assigning functions in chromatin regulation to previously unannotated proteins and suggesting a role for an EELM2 domain-containing protein and a putative microrchidia protein as m

SUBMITTER: Hillier C 

PROVIDER: S-EPMC6693557 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature