Postovulatory ageing modifies sperm-induced Ca²⁺ oscillations in mouse oocytes through a conditions-dependent, multi-pathway mechanism.

ABSTRACT: Postovulatory ageing of mammalian oocytes occurs between their ovulation and fertilization and has been shown to decrease their developmental capabilities. Aged oocytes display numerous abnormalities, including altered Ca²⁺ signalling. Fertilization-induced Ca²⁺ oscillations are essential for activation of the embryonic development, therefore maintaining proper Ca²⁺ homeostasis is crucial for the oocyte quality. In the present paper, we show that the mechanism underlying age-dependent alterations in the pattern of sperm-triggered Ca²⁺ oscillations is more complex and multifaceted than previously believed. Using time-lapse imaging accompanied by immunostaining and molecular analyses, we found that postovulatory ageing affects the amount of Ca

SUBMITTER: Szpila M 

PROVIDER: S-EPMC6694115 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature