A conserved RNA seed-pairing domain directs small RNA-mediated stress resistance in enterobacteria.

ABSTRACT: Small regulatory RNAs (sRNAs) are crucial components of many stress response systems. The envelope stress response (ESR) of Gram-negative bacteria is a paradigm for sRNA-mediated stress management and involves, among other factors, the alternative sigma factor E (σ^E ) and one or more sRNAs. In this study, we identified the MicV sRNA as a new member of the σ^E regulon in Vibrio cholerae. We show that MicV acts redundantly with another sRNA, VrrA, and that both sRNAs share a conserved seed-pairing domain allowing them to regulate multiple target mRNAs. V. cholerae lacking σ^E displayed increased sensitivity toward antimicrobials, and over-expression of either of the sRNAs suppressed this phenotype. Laboratory selection experiments using a library of synthetic s

SUBMITTER: Peschek N 

PROVIDER: S-EPMC6694218 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature