Phylogenetic analysis of clinical strains of Helicobacter pylori isolated from patients with gastric diseases in Tibet.

ABSTRACT:

Background

Helicobacter pylori (H. pylori) is a Gram-negative pathogenic bacterium that causes chronic gastritis and other gastric diseases in humans. In Tibet, China, the infection of H. pylori is an important risk factor that caused gastric cancer.

Methods

To understand the characteristics of this pathogen in Tibet, five strains of H. pylori were isolated from three patients' oral cavity or stomach who had either a gastric ulcer or gastritis. We performed genome sequences of these five clinical strains on Illumina Hiseq, and 55,016-63666 SNVs/InDels were identified by comparing to the reference strain of H. pylori 26995.

Results

The phylogenetic analysis with multi-locus sequence typing (MLST) showed that five Tibetan strains were

SUBMITTER: Bie M 

PROVIDER: S-EPMC6694268 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature