OsDGD2β is the Sole Digalactosyldiacylglycerol Synthase Gene Highly Expressed in Anther, and its Mutation Confers Male Sterility in Rice.

ABSTRACT:

Background

Digalactosyldiacylglycerol (DGDG) is one of the major lipids found predominantly in the photosynthetic membrane of cyanobacteria, eukaryotic algae and higher plants. DGDG, along with MGDG (Monogalactosyldiacylglycerol), forms the matrix in thylakoid membrane of chloroplast, providing the site for photochemical and electron transport reactions of oxygenic photosynthesis.

Results

In silico analysis reveals that rice (Oryza sativa L.) genome has 5 genes encoding DGDG synthase, which are differentially expressed in different tissues, and OsDGD2β was identified to be the sole DGDG synthase gene expressed in anther. We then developed osdgd2β mutants by using the CRISPR/Cas9 system and elucidate its role, especially in the development of anther and pollen. The loss of fu

SUBMITTER: Basnet R 

PROVIDER: S-EPMC6694320 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature