A promising detection candidate for flagellated Salmonella spp.

ABSTRACT: Salmonella is a common and important pathogen for both human and animals. All Salmonella except Salmonella pullorum and Salmonella gallinarum have flagellum. Flagellin (FliC) is the main subunit protein forming the bacterial filament, which is present in large amounts on the surface of all flagellated Salmonella. After bioinformatics analysis, the most highly conserved region (locates position from 1 to 102 amino acid residue of FliC, we named it as FliC') was selected, and corresponding recombinant FliC' (rFliC') protein was tailored as an immunogen to generate monoclonal antibodies (MAbs) against Salmonella flagellin. BALB/c mice were immunized with the purified recombinant protein rFliC', which were prepared by prokaryotic expression system pET22b (+) expressing FliC'. After fusion of s

SUBMITTER: Yang Y 

PROVIDER: S-EPMC6694378 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature