Temporal integration of mitochondrial stress signals by the PINK1:Parkin pathway.

ABSTRACT:

Background

The PINK1:Parkin pathway regulates the autophagic removal of damaged and dysfunctional mitochondria. While the response of this pathway to complete loss of ΔΨm, as caused by high concentrations of mitochondrial ionophores, has been well characterized, it remains unclear how the pathway makes coherent decisions about whether to keep or purge mitochondria in situations where ΔΨm is only partially lost or exhibits fluctuations, as has been observed in response to certain types of cellular stress.

Results

To investigate the responses of the PINK1:Parkin pathway to mitochondrial insults of different magnitude and duration, controlled titration of the mitochondrial protonophore, CCCP, was used to manipulate ΔΨm in live cells, and the dynamics of PINK1 and Parkin recruit

SUBMITTER: Bowling JL 

PROVIDER: S-EPMC6694515 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature