A novel population of extracellular vesicles smaller than exosomes promotes cell proliferation.

ABSTRACT:

Background

Extracellular vesicles (EVs) play important roles in intercellular communication by delivering RNA, lipid, and proteins to neighboring or distant cells. Identification and classification of EVs secreted from diverse cell types are essential for understanding their signaling properties.

Methods

In this study, EVs from the culture media were isolated by ultracentrifugation and analyzed by electron microscopy (EM) and nanoparticle tracking analyses. Conditioned media (CM) from HEK293 cells culture grown either in serum-free (SF) or 10% fetal bovine serum (FBS) containing media were centrifuged at 100,000×g to separate the SN_Δ supernatant and the P100 pellet in which exosomes are enriched. Then, the SN_Δ fraction was centrifuged at 200,000×g to yi

SUBMITTER: Lee SS 

PROVIDER: S-EPMC6694590 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature