The effect of medium supplementation and serial passaging on the transcriptome of human adipose-derived stromal cells expanded in vitro.

ABSTRACT:

Background

For adipose-derived stromal cells (ASCs) to be safe for use in the clinical setting, they need to be prepared using good manufacturing practices (GMPs). Fetal bovine serum (FBS), used to expand ASCs in vitro in some human clinical trials, runs the risk of xenoimmunization and zoonotic disease transmission. To ensure that GMP standards are maintained, pooled human platelet lysate (pHPL) has been used as an alternative to FBS. ASCs proliferate more rapidly in pHPL than in FBS, with no significant change in immunophenotype and differentiation capacity. However, not much is known about how pHPL affects the transcriptome of these cells.

Methods

This study investigated the effect of pHPL and FBS on the ASC transcriptome during in vitro serial expansion from passage 0 to

SUBMITTER: Dessels C 

PROVIDER: S-EPMC6694630 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature