Design of experiments to assess the effect of culture parameters on the osteogenic differentiation of human adipose stromal cells.

ABSTRACT:

Background

Human adipose-derived stromal cells (hASCs) have been gaining increasing popularity in regenerative medicine thanks to their multipotency, ease of collection, and efficient culture. Similarly to other stromal cells, their function is particularly sensitive to the culture conditions, including the composition of the culture medium. Given the large number of parameters that can play a role in their specification, the rapid assessment would be beneficial to allow the optimization of their culture parameters.

Method

Herein we used the design of experiments (DOE) method to rapidly screen the influence and relevance of several culture parameters on the osteogenic differentiation of hASCs. Specifically, seven cell culture parameters were selected for this study based on

SUBMITTER: Kuterbekov M 

PROVIDER: S-EPMC6694725 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature