SWATH-MS screening strategy for the determination of food dyes in spices by UHPLC-HRMS.

ABSTRACT: A multi-class wide-scope screening method for the detection and identification of artificial colours and illegal dyes in spices was developed for regulatory purposes. The screening was carried out by ultra-high performance liquid chromatography hyphenated with a quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS) with sequential window acquisition of all theoretical fragment-ion spectra (SWATH) and was validated with forty-one compounds by spiking experiments in curry and paprika extracts. In order to detect and identify the compounds with a high level of confidence, a home-made tandem mass spectrometry (QTOF-MS/MS) database of approximately one hundred illegal dyes and artificial colours was created. The procedure was then used to screen field samples of spices and spice blends pu

SUBMITTER: Periat A 

PROVIDER: S-EPMC6694849 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature