The Human G Protein-Coupled ATP Receptor P2Y₁₁ Is Associated With IL-10 Driven Macrophage Differentiation.

ABSTRACT: The G protein-coupled P2Y₁₁ receptor is known to sense extracellular ATP during inflammatory and immune responses. The dinucleotide NAD⁺ has also been proposed to be a P2Y₁₁ receptor ligand but its role is less clear. Here, we have examined for the first time human P2Y₁₁ receptor protein levels and show that the receptor was upregulated during polarization of M2 macrophages. IL-10 reinforced P2Y₁₁ receptor expression during differentiation of M2c macrophages expressing CD163, CD16, and CD274 (PD-L1). Nutlin-3a mediated p53 stabilization further increased P2Y₁₁ receptor, CD16, and PD-L1 expression. AMP-activated kinase (AMPK), which mediates anti-inflammatory effects of IL-10, and nicotinamide phosphoribosyltransferase (NAMPT

SUBMITTER: Gruenbacher G 

PROVIDER: S-EPMC6695557 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature