Early Sex Differences in the Immune-Inflammatory Responses to Neonatal Ischemic Stroke.

ABSTRACT: We recently reported that neonatal ischemia induces microglia/macrophage activation three days post-ischemia. We also found that female mice sustained smaller infarcts than males three months post-ischemia. The objective of our current study was to examine whether differential acute neuroinflammatory response and infiltrated immune cells occurs between male and females after three days post-ischemia. Permanent middle cerebral artery occlusion was induced in male and female postnatal 9-day-old (P9) mice, and mice were sacrificed three days after ischemia. Brains were analyzed for mRNA transcription after microglia magnetic cell sorting to evaluate M1 and M2 markers. FACS analysis was performed to assess myeloid infiltration and microglial expression of CX3 chemokine receptor 1 (CX3CR1). Inf

SUBMITTER: Villapol S 

PROVIDER: S-EPMC6695584 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature