Magnetized Carbon Nanotube Based Lateral Flow Immunoassay for Visual Detection of Complement Factor B.

ABSTRACT: The authors describe a magnetized carbon nanotube (MCNT) based lateral flow immunoassay (LFI) for visual detection of complement factor B (CFB) in blood. MCNT was prepared by decorating magnetic Fe₃O₄ nanoparticles on multi-walled CNT surface and used as a colored tag for LFI. Monoclonal antibody (mAb, Ab₁) of CFB was covalently immobilized on the MCNT surface via diimide-activated conjugation between the carboxyl groups on the MCNT surface and amino groups of antibodies. Polyclonal antibody of CFB (Ab₂) and the secondary antibody were used to prepare the lateral flow test strips. The assay involved: (1) the capture of CFB in blood with the mAb-functionalized MCNT; (2) magnetic separation of the formed CFB-mAb-MCNT and excess of mAb-MCNT from the

SUBMITTER: Huang Y 

PROVIDER: S-EPMC6695926 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature