MAS NMR on a Red/Far-Red Photochromic Cyanobacteriochrome All2699 from Nostoc.

ABSTRACT: Unlike canonical phytochromes, the GAF domain of cyanobacteriochromes (CBCRs) can bind bilins autonomously and is sufficient for functional photocycles. Despite the astonishing spectral diversity of CBCRs, the GAF1 domain of the three-GAF-domain photoreceptor all2699 from the cyanobacterium Nostoc 7120 is the only CBCR-GAF known that converts from a red-absorbing (Pr) dark state to a far-red-absorbing (Pfr) photoproduct, analogous to the more conservative phytochromes. Here we report a solid-state NMR spectroscopic study of all2699g1 in its Pr state. Conclusive NMR evidence unveils a particular stereochemical heterogeneity at the tetrahedral C3¹ atom, whereas the crystal structure shows exclusively the R-stereochemistry at this chiral center. Additional NMR experim

SUBMITTER: Xu QZ 

PROVIDER: S-EPMC6696110 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature