Thyroid Peroxidase Activity is Inhibited by Phenolic Compounds-Impact of Interaction.

ABSTRACT: The aim of this study was to estimate the mode of thyroid peroxidase (TPO) inhibition by polyphenols: Chlorogenic acid, rosmarinic acid, quercetin, and rutin. All the tested polyphenols inhibited TPO; the IC₅₀ values ranged from 0.004 mM to 1.44 mM (for rosmarinic acid and rutin, respectively). All these pure phytochemical substances exhibited different modes of TPO inhibition. Rutin and rosmarinic acid showed competitive, quercetin-uncompetitive and chlorogenic acid-noncompetitive inhibition effect on TPO. Homology modeling was used to gain insight into the 3D structure of TPO and molecular docking was applied to study the interactions of the inhibitors with their target at the molecular level. Moreover, the type and strength of mutual interactions between the inhibitors (expre

SUBMITTER: Habza-Kowalska E 

PROVIDER: S-EPMC6696198 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature