Evaluating the Efficiency of gRNAs in CRISPR/Cas9 Mediated Genome Editing in Poplars.

ABSTRACT: CRISPR/Cas9 has become one of the most promising techniques for genome editing in plants and works very well in poplars with an Agrobacterium-mediated transformation system. We selected twelve genes, including SOC1, FUL, and their paralogous genes, four NFP-like genes and TOZ19 for three different research topics. The gRNAs were designed for editing, and, together with a constitutively expressed Cas9 nuclease, transferred either into the poplar hybrid Populus × canescens or into P. tremula. The regenerated lines showed different types of editing and revealed several homozygous editing events which are of special interest in perennial species because of limited back-cross ability. Through a time series, we could show that despite the constitutive expression of the Cas9 nuclease, no secondar

SUBMITTER: Bruegmann T 

PROVIDER: S-EPMC6696231 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature