Lysine methyltransferase 2D regulates pancreatic carcinogenesis through metabolic reprogramming.

ABSTRACT:

Objective

Despite advances in the identification of epigenetic alterations in pancreatic cancer, their biological roles in the pathobiology of this dismal neoplasm remain elusive. Here, we aimed to characterise the functional significance of histone lysine methyltransferases (KMTs) and demethylases (KDMs) in pancreatic tumourigenesis.

Design

DNA methylation sequencing and gene expression microarrays were employed to investigate CpG methylation and expression patterns of KMTs and KDMs in pancreatic cancer tissues versus normal tissues. Gene expression was assessed in five cohorts of patients by reverse transcription quantitative-PCR. Molecular analysis and functional assays were conducted in genetically modified cell lines. Cellular metabolic rates were measured using an XF24

SUBMITTER: Koutsioumpa M 

PROVIDER: S-EPMC6697184 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature